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Toxicity of the bacterial luciferase substrate, n ‐decyl aldehyde, to Saccharomyces cerevisiae and Caenorhabditis elegans
Author(s) -
Hollis R.P.,
Lagido C.,
Pettitt J.,
Porter A.J.R.,
Killham K.,
Paton G.I.,
Glover L.A.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02905-2
Subject(s) - luciferase , saccharomyces cerevisiae , caenorhabditis elegans , escherichia coli , reporter gene , biology , bioassay , in vivo , biochemistry , gene , bacteria , microbiology and biotechnology , chemistry , gene expression , genetics , transfection
This study determined that the bacterial luciferase fusion gene ( lux AB) was not a suitable in vivo gene reporter in the model eukaryotic organisms Saccharomyces cerevisiae and Caenorhabditis elegans . Lux AB expressing S. cerevisiae strains displayed distinctive rapid decays in luminescence upon addition of the bacterial luciferase substrate, n ‐decyl aldehyde, suggesting a toxic response. Growth studies and toxicity bioassays have subsequently confirmed, that the aldehyde substrate was toxic to both organisms at concentrations well tolerated by Escherichia coli . As the addition of aldehyde is an integral part of the bacterial luciferase activity assay, our results do not support the use of lux reporter genes for in vivo analyses in these model eukaryotic organisms.