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Diminished corneal angiogenesis in gelatinase A‐deficient mice
Author(s) -
Kato Takuji,
Kure Tomoko,
Chang Jin-Hong,
Gabison Eric E.,
Itoh Takeshi,
Itohara Shigeyoshi,
Azar Dimitri T.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02897-6
Subject(s) - gelatinase , angiogenesis , gelatinase a , neovascularization , endocrinology , medicine , wild type , chemistry , matrix metalloproteinase , biology , biochemistry , gene , mutant
The goal of the present study was to define the role of gelatinase A in angiogenesis. We performed corneal micropocket assays in gelatinase A‐deficient mice and their age‐matched wild‐type littermates. The corneal neovascular area in gelatinase A‐deficient mice (0.15±0.14 mm 2 ) was significantly less than that of wild‐type littermates (0.53±0.35 mm 2 ; P <0.01). Similarly, aortic ring assays showed significant reduction of endothelial outgrowth in gelatinase A‐deficient mice (0.26±0.14 mm 2 ) as compared to wild‐type littermates (0.44±0.06 mm 2 ; P <0.05). These results suggest that gelatinase A may play an important role in the regulation of corneal angiogenesis.