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Signaling pathways utilized by tumor necrosis factor receptor 1 in adipocytes to suppress differentiation
Author(s) -
Xu Haiyan,
Hotamisligil Gökhan S.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02889-7
Subject(s) - adipogenesis , tumor necrosis factor alpha , adipocyte , microbiology and biotechnology , intracellular , tumor necrosis factor receptor 1 , receptor , transmembrane protein , signal transduction , psychological repression , biology , chemistry , endocrinology , adipose tissue , tumor necrosis factor receptor , biochemistry , gene , gene expression , mesenchymal stem cell
Tumor necrosis factor‐α (TNFα) has profound effects on cultured adipocytes, one of which is the inhibition of terminal differentiation. Previous studies in TNF receptor (TNFR)‐deficient preadipocytes have demonstrated that the anti‐adipogenic effect of both secreted and transmembrane TNFα is mediated solely by TNFR1. In this study, we performed a structure–function analysis of the intracellular domains of TNFR1 and investigated the signaling pathway(s) involved in TNFR1‐mediated inhibition of adipocyte differentiation. Our results show that repression of adipogenesis required the juxtamembrane and death domains and was independent of the pathways involving nuclear factor κB and neutral sphingomyelinase.