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The potato tuber transcriptome: analysis of 6077 expressed sequence tags
Author(s) -
Crookshanks Meg,
Emmersen Jeppe,
Welinder Karen G.,
Lehmann Nielsen Kåre
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02888-5
Subject(s) - solanum tuberosum , biology , stolon , gene , transcriptome , complementary dna , cdna library , starch , expressed sequence tag , botany , sequence analysis , homology (biology) , genetics , gene expression , biochemistry
This is the first report of the biosynthetic potential of a tuber storage organ investigated by expressed sequence tag sequencing. A cDNA library was generated from the mature tuber of field grown potato ( Solanum tuberosum var. Kuras). Partial sequences obtained from 6077 clones were assembled into 828 clusters and 1533 singletons. The average read length was 592 bp, and 2254 clones were full length. 5717 clones showed homology to genes from other organisms. Genes involved in protein synthesis, protein destination and cell defense predominated in tuber compared to stolon, shoot and leaf organs. 1063 clones were unique to tuber. Transcripts of starch metabolizing enzymes showed similar relative levels in tuber and stolon.