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Nitric oxide production in living neurons is modulated by sphingosine: a fluorescence microscopy study
Author(s) -
Viani Paola,
Giussani Paola,
Ferraretto Anita,
Signorile Alessandra,
Riboni Laura,
Tettamanti Guido
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02880-0
Subject(s) - nitric oxide , sphingosine , ceramide , chemistry , microbiology and biotechnology , fluorescence microscope , granule (geology) , biophysics , sphingosine 1 phosphate , biochemistry , fluorescence , biology , receptor , apoptosis , paleontology , physics , organic chemistry , quantum mechanics
An investigation was carried out into the possible effect of sphingosine (Sph) on nitric oxide (NO) production in living neurons. Differentiated granule cells were used in a dynamic videoimaging analysis of single cells labeled, simultaneously, with FURA‐2 and the NO indicator 4,5‐diaminofluorescein. The results demonstrate that Sph exerts a potent inhibitory effect on the Ca 2+ ‐dependent production of NO, without modifying the [Ca 2+ ] i . The effect appears to be specific as neither ceramide nor Sph‐1‐phosphate had any effect on the NO and [Ca 2+ ] i levels. The data demonstrate that Ca 2+ ‐dependent NO production is a specific Sph target in living granule cells, suggesting that this bioactive sphingoid plays a relevant role in neuronal NO signaling.