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‘Empty’ minicircles and petB/atpA and psbD/psbE (cyt b 559 α) genes in tandem in Amphidinium carterae plastid DNA1
Author(s) -
Hiller Roger G.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02871-x
Subject(s) - minicircle , gene , dinoflagellate , genetics , biology , microbiology and biotechnology , botany
Amphidinium carterae minicircle chloroplast DNA was separated from total DNA by centrifugation through a sucrose/NaCl gradient. Sequences of minicircles with psbA and 23S rRNA contained a common region of 67 bp. Primers designed from this generated numerous polymerase chain reaction products of 1.5–2.6 kb. These contained psaA and psaB as one gene/circle, and petB/atpA and psbD/psbE as two genes/circle. ‘Empty’ minicircles of 1.7–2.5 kb containing no identifiable genes or parts of genes were more abundant than gene‐containing circles. From 15 minicircles a minimum common region of 48 bp was identified, with little identity to that from other dinoflagellate minicircles.