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Direct observation of membrane retrieval in chromaffin cells by capacitance measurements
Author(s) -
Henkel Andreas W,
Horstmann Heinz,
Henkel M.Kerstin
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02861-7
Subject(s) - endocytic cycle , vesicle , organelle , membrane , exocytosis , electron microscope , biophysics , endocytosis , chemistry , microbiology and biotechnology , biology , cell , biochemistry , optics , physics
This study was focussed on the identification of the endocytic organelles in chromaffin cells which retrieve large, dense core vesicle (LDCV)‐membrane components from the plasma membrane. For this purpose, ‘on‐cell’ capacitance measurements and electron microscopy were employed. We found capacitance steps and capacitance flickers, corresponding to single exo‐ and endocytic events. The analysis revealed that the total membrane surface of completely fused LDCVs is recycled by large endocytic vesicles and smaller, most likely clathrin‐coated vesicles, at approximately the same ratio. These results were confirmed by rapid‐freeze immuno‐electron microscopy, where an extracellular marker was rapidly internalized into endocytic vesicles that morphologically resembled LDCVs.