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Replication of the rat aldolase B locus differs between aldolase B‐expressing and non‐expressing cells
Author(s) -
Miyagi Satoru,
Zhao Yung-peng,
Saitoh Yasushi,
Tamai Katsuyuki,
Tsutsumi Ken-ichi
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02849-6
Subject(s) - aldolase a , biology , dna replication , microbiology and biotechnology , bromodeoxyuridine , transcription (linguistics) , replication factor c , gene , dna , primer (cosmetics) , eukaryotic dna replication , genetics , chemistry , enzyme , biochemistry , cell growth , linguistics , philosophy , organic chemistry
We previously reported a rat chromosomal origin of DNA replication (oriA1) that encompassed the aldolase B (AldB) gene promoter. Here, we examined utilization of oriA1 in AldB‐expressing and non‐expressing cells. The results suggested the occurrence of mutually exclusive regulation between DNA replication and transcription. Nascent strand abundance as assayed by competitive polymerase chain reaction using bromodeoxyuridine‐labeled nascent DNA indicated that oriA1 is not utilized in AldB‐expressing cells, while it is fired in non‐expressing cells. In the latter non‐expressing cells, the replication fork seemed to slow at 20–22 kb downstream of oriA1.