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Penetratin‐induced aggregation and subsequent dissociation of negatively charged phospholipid vesicles
Author(s) -
Persson Daniel,
Thorén Per E.G,
Nordén Bengt
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02843-5
Subject(s) - phospholipid , vesicle , chemistry , dissociation (chemistry) , biophysics , biochemistry , membrane , organic chemistry , biology
The interaction of the cellular delivery vector penetratin with a model system consisting of negatively charged phospholipid vesicles has been studied. Above a certain peptide to lipid molar ratio, the cationic oligopeptide induces vesicle aggregation. Interestingly, the aggregation is followed by spontaneous disaggregation, which may be related to membrane translocation of the peptide. Circular dichroism (CD) measurements indicate a conformational transition, from α‐helix to antiparallel β‐pleated sheet, which is simultaneous with the aggregation process. The potential influence of spectroscopic artifacts on CD data due to the drastically increased turbidity during aggregation is discussed.