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Role of the sequence of the rne‐dependent site in 3′ processing of M1 RNA, the catalytic component of Escherichia coli RNase P
Author(s) -
Sim Soyeong,
Kim Semi,
Lee Younghoon
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02822-8
Subject(s) - rnase p , cleavage (geology) , rna , escherichia coli , biology , ribonuclease iii , downstream processing , chemistry , biochemistry , microbiology and biotechnology , gene , rna interference , paleontology , fracture (geology)
The 3′ processing of M1 RNA, the catalytic component of Escherichia coli RNase P, occurs by two pathways involving multiple steps. The precursor of M1 RNA has an rne‐dependent site downstream of the processing site, whose sequence variation affects the processing efficiency. In this study, we showed that the sequence itself of the rne‐dependent site possessed the ability to determine the processing pathways and that it also affected the cleavage specificity with the generation of the processing products at one nucleotide upstream or downstream of the normal cleavage sites.