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A DNA polymerase ϵ inhibitor activates the ribo and deoxyribo modes of primase expression and induces a unique phenomenon of primer accumulation1
Author(s) -
Řı́man Josef
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02808-3
Subject(s) - primase , dna polymerase , primer (cosmetics) , polymerase , microbiology and biotechnology , dna , biology , dna replication , processivity , biochemistry , chemistry , polymerase chain reaction , gene , reverse transcriptase , organic chemistry
Carbonyldiphosphonate (COMDP), a selective inhibitor of DNA polymerase (pol) ϵ, strongly stimulates expression of the ribo and deoxyribo modes of primase (Pr) activities of the Pr–DNA pol α enzyme complex associated with special cytoplasmic nucleoprotein complexes of chicken leukemic myeloblasts [J. Řı́man and A. Šulová, Acta Virol. 41 (1997) 181–214]. Besides stimulation, COMDP uncouples the Pr activities from those of DNA pol α, inducing in this way a unique phenomenon of accumulation of primers of basic length. In the presence of dNTPs, the COMDP effect is counteracted by excess of mimosine. The mutually exclusive effects of these agents are discussed.