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D1′ centers are less efficient than normal photosystem II centers
Author(s) -
Funk Christiane,
Wiklund Ronney,
Schröder Wolfgang P.,
Jansson Christer
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02794-6
Subject(s) - photosystem ii , p680 , photosynthetic reaction centre , chlorophyll fluorescence , chemistry , yield (engineering) , photosynthesis , photochemistry , oxygen evolution , chlorophyll , kinetics , mutant , fluorescence , quantum yield , synechocystis , recombination , chlorophyll a , biochemistry , photosystem i , materials science , physics , gene , optics , organic chemistry , electrode , quantum mechanics , metallurgy , electrochemistry
One prominent difference between the photosystem II (PSII) reaction center protein D1′ in Synechocystis 6803 and normal D1 is the replacement of Phe‐186 in D1 with leucine in D1′. Mutants of Synechocystis 6803 producing only D1′, or containing engineered D1 proteins with Phe‐186 substitutions, were analyzed by 77 K fluorescence emission spectra, chlorophyll a fluorescence induction yield and decay kinetics, and flash‐induced oxygen evolution. Compared to D1‐containing PSII centers, D1′ centers exhibited a 50% reduction in variable chlorophyll a fluorescence yield, while the flash‐induced O 2 evolution pattern was unaffected. In the F186 mutants, both the P680 + /Q A − recombination and O 2 oscillation pattern were noticeably perturbed.