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Differential display analysis of mutants for the transcription factor Pdr1p regulating multidrug resistance in the budding yeast
Author(s) -
Miura Fumihito,
Yada Tetsushi,
Nakai Kenta,
Sakaki Yoshiyuki,
Ito Takashi
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02792-2
Subject(s) - biology , open reading frame , gene , mutant , transcription factor , saccharomyces cerevisiae , differential display , yeast , genetics , transcription (linguistics) , multiple drug resistance , transcriptome , context (archaeology) , microbiology and biotechnology , drug resistance , gene expression , paleontology , linguistics , philosophy , peptide sequence
The transcription factor Pdr1p recognizes Pdr1p/Pdr3p‐response element (PDRE) to activate genes involved in multidrug resistance of the budding yeast. To identify novel targets of Pdr1p, we compared transcriptomes among the yeast cells bearing wild, disrupted and gain‐of‐function alleles of PDR1 using a high‐throughput fluorescent differential display PCR. Consequently, we identified 20 transcripts apparently regulated by Pdr1p, which are derived from well‐known target genes as well as those that have never been described in the context of drug resistance. Intriguingly, among the latter, a previously unrecognized gene bearing a small putative open reading frame preceded by a functional PDRE was found.