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FKBP12 associates tightly with the skeletal muscle type 1 ryanodine receptor, but not with other intracellular calcium release channels
Author(s) -
Carmody Mark,
Mackrill John J.,
Sorrentino Vincenzo,
O'Neill Cora
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02787-9
Subject(s) - ryanodine receptor , ryr1 , skeletal muscle , ryanodine receptor 2 , fkbp , gene isoform , cardiac muscle , calcineurin , chemistry , microsome , inositol trisphosphate receptor , myocyte , biochemistry , receptor , biology , inositol , endocrinology , microbiology and biotechnology , medicine , in vitro , transplantation , gene
This study compared the relative levels of ryanodine receptor (RyR) isoforms, inositol 1,4,5‐trisphosphate receptor (IP 3 R) isoforms, and calcineurin, plus their association with FKBP12 in brain, skeletal and cardiac tissue. FKBP12 demonstrated a very tight, high affinity association with skeletal muscle microsomes, which was displaced by FK506. In contrast, FKBP12 was not tightly associated with brain or cardiac microsomes and did not require FK506 for removal from these organelles. Furthermore, of the proteins solubilised from skeletal muscle, cardiac muscle and brain microsomes, only skeletal muscle RyR1 bound to an FKBP12–glutathione‐ S ‐transferase fusion protein, in a high affinity FK506 displaceable manner. These results suggest that RyR1 has distinctive FKBP12 binding properties when compared to RyR2, RyR3, all IP 3 R isoforms and calcineurin.