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Requirement of ceramide for adhesion of Helicobacter pylori to glycosphingolipids
Author(s) -
Tang Wei,
Seino Keita,
Ito Mieko,
Konishi Toshiro,
Senda Hisato,
Makuuchi Masatoshi,
Kojima Naoya,
Mizuochi Tsuguo
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02759-4
Subject(s) - ceramide , chemistry , glycosphingolipid , biochemistry , helicobacter pylori , galactose , adhesion , fatty acid , sphingomyelin , glycolipid , conjugated linoleic acid , lactose , bacteria , biology , organic chemistry , cholesterol , linoleic acid , apoptosis , genetics
Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaccharide‐conjugated neoglycoconjugates in order to clarify the roles of the carbohydrate and lipid portions of GSLs in H. pylori adhesion. These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to ceramide, sphingomyelin, or polyacrylamides conjugated with β‐galactose, lactose, 3′‐sialyllactose or 3′‐sulfo‐β‐galactose. Furthermore, neoglycolipids or bovine serum albumin derivatives with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with α‐hydroxyl fatty acid was much stronger than GalCer with the non‐hydroxyl fatty acid. These results suggest that H. pylori recognize the conformation of GSL with α‐hydroxyl fatty acid on solid phase.