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Probing into the function of the gene product responsible for glycogen storage disease type Ib
Author(s) -
Xie Wensheng,
van de Werve Gérald,
Berteloot Alfred
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02758-2
Subject(s) - phosphate , biochemistry , glucose 6 phosphate , chemistry , vanadate , hexose , efflux , glycogen storage disease type i , enzyme , biology , glycogen storage disease , glycogen
This study aimed at directly assessing glucose 6‐phosphate (G6P) transport by intact rat liver microsomes. Tracer uptake from labeled G6P occurred with T 1/2 values that proved insensitive to unlabeled G6P or 100 μM vanadate, and could not be activated over background levels by intravesicular phosphate in the complete absence of G6P hydrolysis. [ 32 P]Phosphate efflux was similarly unaffected by G6P or phosphate in the incubation medium. We conclude that the gene product responsible for glycogen storage disease type Ib is functionally distinct from the bacterial hexose phosphate transporter, which operates as an obligatory phosphate:phosphate or G6P:phosphate exchanger.