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Neurophilin‐1 is a downstream target of transcription factor Ets‐1 in human umbilical vein endothelial cells
Author(s) -
Teruyama Kazuhide,
Abe Mayumi,
Nakano Toru,
Takahashi Shoki,
Yamada Shogo,
Sato Yasufumi
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02724-7
Subject(s) - umbilical vein , angiogenesis , neuropilin 1 , blot , messenger rna , transcription factor , microbiology and biotechnology , biology , chemistry , gene , vascular endothelial growth factor , cancer research , biochemistry , vegf receptors , in vitro
Transcription factor Ets‐1 expressed in endothelial cells promotes angiogenesis. Here, we transiently overexpressed Ets‐1 in human umbilical vein endothelial cells (HUVECs) and comprehensively searched for potential downstream targets of Ets‐1 by cDNA microarray analysis. The expression of several angiogenesis‐related genes including neuropilin‐1 was augmented by the overexpression of Ets‐1. Quantitative real‐time RT‐PCR and Western blotting confirmed the increase in the levels of neuropilin‐1 mRNA and protein. In contrast, dominant negative ets‐1 decreased the levels of neuropilin‐1 mRNA and protein. These results indicate that neuropilin‐1 is a downstream target of Ets‐1 in HUVECs.