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mik1 + G1–S transcription regulates mitotic entry in fission yeast
Author(s) -
Ng Szu Shien,
Anderson Mark,
White Stewart,
McInerny Christopher J
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02720-x
Subject(s) - schizosaccharomyces pombe , mitosis , cyclin dependent kinase 1 , schizosaccharomyces , biology , yeast , microbiology and biotechnology , cell cycle , transcription (linguistics) , gene , saccharomyces cerevisiae , genetics , linguistics , philosophy
In the fission yeast Schizosaccharomyces pombe Mik1p, in combination with Wee1p, is an important inhibitor of mitosis through direct phosphorylation of Cdc2p. Here we present the observation that mik1 + is transcribed during G1‐ and S‐phase in normally dividing cells. mik1 + transcription is regulated by the MCB–DSC1 system, which controls expression of other genes at the G1–S interval. mik1 + is shown to be an important target of MCB–DSC1 as it is epistatic for the mitotic delay phenotype displayed in cdc10‐C4 cells, which are mutated in a component of DSC1. The mitotic delay in cdc10‐C4 cells is bypassed by cdc2‐1w , suggesting that mik1 + acts directly on cdc2 + , with no checkpoint function involved. Thus, mik1 + represents a new type of MCB–DSC1 regulated gene in fission yeast, whose gene product is exclusively expressed during G1‐ and S‐phase to prevent premature mitosis during this cell cycle stage.