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Efficient biochemical engineering of cellular sialic acids using an unphysiological sialic acid precursor in cells lacking UDP‐ N ‐acetylglucosamine 2‐epimerase
Author(s) -
Mantey Lars R,
Keppler Oliver T,
Pawlita Michael,
Reutter Werner,
Hinderlich Stephan
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02701-6
Subject(s) - sialic acid , glycoconjugate , biochemistry , chemistry , n acetylneuraminic acid , population , demography , sociology
Sialic acids comprise a family of terminal sugars essential for a variety of biological recognition systems. N ‐Propanoylmannosamine, an unphysiological sialic acid precursor, is taken up and metabolized by mammalian cells resulting in oligosaccharide‐bound N ‐propanoylneuraminic acid. N ‐Propanoylmannosamine, applied to endogenously hyposialylated subclones of the myeloid leukemia HL60 and of the B‐cell lymphoma BJA‐B, both deficient in UDP‐ N ‐acetylglucosamine 2‐epimerase, is efficiently metabolized to CMP‐ N ‐propanoylneuraminic acid resulting in up to 85% of glycoconjugate‐associated sialic acids being unphysiological N ‐propanoylneuraminic acid. Thus, UDP‐ N ‐acetylglucosamine 2‐epimerase‐deficient cell lines provide an important experimental progress in engineering cells to display an almost homogeneous population of defined, structurally altered sialic acids.