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Activation of c‐Jun amino‐terminal kinase is required for retinoic acid‐induced neural differentiation of P19 embryonal carcinoma cells
Author(s) -
Wang Hong,
Ikeda Shoko,
Kanno Shin-ichiro,
Guang Li Ming,
Ohnishi Motoko,
Sasaki Masato,
Kobayashi Takayasu,
Tamura Shinri
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02699-0
Subject(s) - okadaic acid , p19 cell , retinoic acid , protein kinase r , mitogen activated protein kinase kinase , chemistry , protein kinase a , map2k7 , phosphatase , kinase , ask1 , microbiology and biotechnology , cellular differentiation , cyclin dependent kinase 2 , biochemistry , biology , phosphorylation , gene , adult stem cell
P19 embryonal carcinoma cells are known to differentiate into neurons and glia when treated with relatively high concentrations (>100 nM) of retinoic acid (RA). Concomitant with this RA‐induced neural differentiation, we observed an activation of the c‐Jun amino‐terminal kinase (JNK). JNK was required for the RA‐induced neural differentiation, because dominant‐negative JNK blocked the differentiation. Studies using protein phosphatase inhibitors and protein kinase inhibitors suggested that both okadaic acid‐sensitive protein phosphatase(s) and protein kinase C participate in the RA‐induced activation of JNK.