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Stimulation of nuclear sphingosine kinase activity by platelet‐derived growth factor
Author(s) -
Kleuser Burkhard,
Maceyka Michael,
Milstien Sheldon,
Spiegel Sarah
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02697-7
Subject(s) - sphingosine , sphingosine kinase , lipid signaling , nucleoplasm , microbiology and biotechnology , biology , sphingosine 1 phosphate , biochemistry , kinase , chemistry , nucleus , enzyme , receptor , nucleolus
Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine‐1‐phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet‐derived growth factor, in addition to rapidly stimulating cytosolic sphingosine kinase, also induced a large increase in nucleoplasm‐associated activity after 12–24 h that correlated with progression of cells to the S‐phase of the cell cycle and translocation of sphingosine kinase–green fluorescent protein fusion protein to the nuclear envelope. Our results add sphingosine kinase to the growing list of lipid‐metabolizing enzymes associated with the nucleus, and suggest that sphingosine‐1‐phosphate may also play a role in signal transduction in the nucleus.