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Sequence‐specific binding property of Arabidopsis thaliana telomeric DNA binding protein 1 (AtTBP1)
Author(s) -
Hwang Moo Gak,
Chung In Kwon,
Kang Bin Goo,
Cho Myeon Haeng
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02685-0
Subject(s) - biology , arabidopsis , telomere binding protein , arabidopsis thaliana , gene , myb , dna , complementary dna , telomere , genetics , dna binding domain , dna binding protein , homology (biology) , binding domain , microbiology and biotechnology , binding site , mutant , gene expression , transcription factor
We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1 , encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single‐copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a single Myb telomeric DNA binding domain at the C‐terminus and an extensive homology with other known telomere‐binding proteins. The isolated C‐terminus of AtTBP1 is capable of sequence‐specific DNA binding to plant duplex telomeric DNA. These results suggest that AtTBP1 may play important roles in plant telomere function in vivo.