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Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress‐induced premature senescence
Author(s) -
Dumont Patrick,
Royer Véronique,
Pascal Thierry,
Dierick Jean-François,
Chainiaux Florence,
Frippiat Christophe,
de Magalhaes Joao Pedro,
Eliaers François,
Remacle José,
Toussaint Olivier
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02679-5
Subject(s) - telomere , senescence , oxidative stress , ploidy , stress (linguistics) , wi 38 , andrology , population , biology , cell growth , cell , kinetics , microbiology and biotechnology , genetics , chemistry , endocrinology , dna , medicine , gene , linguistics , philosophy , environmental health , physics , quantum mechanics
WI‐38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 μM tert ‐butylhydroperoxide (t‐BHP) stresses, with a stress at every two population doublings (PD). After subcytotoxic 160 μM H 2 O 2 stress or five repeated 30 μM t‐BHP stresses along the same PD, respectively a 322±55 and 380±129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress.