Premium
Transcriptional effect of hypoxia on placental leptin
Author(s) -
Grosfeld Alexandra,
Turban Sophie,
André Jocelyne,
Cauzac Michèle,
Challier Jean-Claude,
Hauguel-de Mouzon Sylvie,
Guerre-Millo Michèle
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02673-4
Subject(s) - leptin , hypoxia (environmental) , endocrinology , medicine , preeclampsia , biology , trophoblast , messenger rna , placenta , chemistry , gene , genetics , fetus , pregnancy , obesity , oxygen , organic chemistry
We observed recently1 that placental leptin is markedly increased in preeclampsia. Since this disorder is associated with vascular disorders, we have tested the hypothesis that hypoxia regulates leptin expression. We show that hypoxia increased leptin mRNA and secretion in trophoblast‐derived BeWo cells. This effect was mediated through leptin promoter activation. 5′ deletion analysis allowed us to delineate two regions containing 1.87 kb and 1.20 kb of the promoter which conferred respectively high and low responsiveness to hypoxia. These data indicate that leptin is up‐regulated by hypoxia through a transcriptional mechanism likely to involve distinct hypoxia‐responsive cis ‐acting sequences on the promoter.