Premium
Identification of rod‐ and cone‐specific phosducins in teleost retinas
Author(s) -
Kobayashi Yuko,
Hisatomi Osamu,
Satoh Takunori,
Tokunaga Fumio
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02670-9
Subject(s) - oryzias , visual phototransduction , retina , biology , vertebrate , in situ hybridization , microbiology and biotechnology , adaptation (eye) , arrestin , anatomy , genetics , neuroscience , messenger rna , gene , signal transduction , g protein coupled receptor
Phosducin (PD) is a regulatory protein of vertebrate phototransduction cascades. In mammalian retina, it has been thought that only one kind of PD commonly exists in both rods and cones. However, we have found two kinds of PD (OlPD‐R and OlPD‐C) in the retina of a teleost, medaka ( Oryzias latipes ). In situ hybridization and immunohistochemical analysis demonstrated that OlPD‐R and ‐C are selectively expressed in rods and cones, respectively. The antiserum against medaka PDs recognized two kinds of proteins in bluegill ( Lepomis macrochirus ) retina. These results suggest that rod‐ and cone‐specific PDs exist in teleost retinas, probably creating differences in light adaptation between rods and cones.