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Exon 3 of the α folate receptor gene contains a 5′ splice site which confers enhanced ovarian carcinoma specific expression
Author(s) -
Galmozzi Enrico,
Tomassetti Antonella,
Sforzini Sabrina,
Mangiarotti Fabio,
Mazzi Mimma,
Nachmanoff Kiki,
Elwood Patrick C.,
Canevari Silvana
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02659-x
Subject(s) - exon , chloramphenicol acetyltransferase , ovarian carcinoma , microbiology and biotechnology , biology , rna splicing , gene , promoter , alternative splicing , folate receptor , splice , cancer research , reporter gene , gene expression , ovarian cancer , genetics , rna , cancer , cancer cell
The human folate receptor (FR) is overexpressed in ovarian carcinoma. FR transcripts are heterogeneous due to the use of two promoters, P1 and P4, and alternative splicing of exon 3. RNase protection assay and RT‐PCR revealed higher levels of the transcripts that include exon 3 in lines and specimens from ovarian carcinoma. A P1–chloramphenicol acetyltransferase (CAT) construct containing exon 3 demonstrated efficient reporter expression only in ovarian carcinoma. 5′ and 3′ deleted variants of the P1–CAT construct were analyzed by RT‐PCR of the exogenous transcripts and reporter activity. A 5′ splice site and 35 bp downstream intronic region of exon 3 appeared to regulate enhanced FR expression in ovarian carcinoma.