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Activation of Raf‐1 is defective in annexin 6 overexpressing Chinese hamster ovary cells
Author(s) -
Pons Mònica,
Tebar Francesc,
Kirchhoff Matthias,
Peiró Sandra,
de Diego Iñaki,
Grewal Thomas,
Enrich Carlos
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02635-7
Subject(s) - chinese hamster ovary cell , annexin a2 , annexin , cell culture , microbiology and biotechnology , protein kinase c , chemistry , hamster , protein kinase a , epidermal growth factor , cell growth , biology , kinase , cell , biochemistry , receptor , genetics
Annexin 6 is a Ca 2+ ‐dependent phospholipid‐binding protein involved in membrane trafficking. In this study we demonstrate the association of Raf‐1 with recombinant rat annexin 6. Raf–annexin 6 interaction was shown to be independent of cell activation by epidermal growth factor (EGF) or phorbol esters (12‐ O ‐tetradecanoyl‐phorbol‐13‐acetate (TPA)). A stable Chinese hamster ovary (CHO)‐anx6 cell line overexpressing annexin 6 was established to examine the function of annexin 6. In these cells, no increase of Ras‐GTP levels, induced by EGF or TPA, was detected. In addition, the activity of Raf was completely inhibited, whereas the mitogen‐activated protein kinase‐P was unaffected.