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Increased expression of mitochondrial transcription factor A and nuclear respiratory factor‐1 in skeletal muscle from aged human subjects
Author(s) -
Lezza Angela M.S.,
Pesce Vito,
Cormio Antonella,
Fracasso Flavio,
Vecchiet Jacopo,
Felzani Giorgio,
Cantatore Palmiro,
Gadaleta Maria Nicola
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02628-x
Subject(s) - transcription factor , skeletal muscle , mitochondrion , nrf1 , biology , medicine , microbiology and biotechnology , endocrinology , genetics , gene
The expression of two factors involved in the nuclear–mitochondrial crosstalk, namely the mitochondrial transcription factor A (TFAM) and the nuclear respiratory factor‐1 (NRF‐1), was studied in human skeletal muscle biopsies of young and aged subjects. Aged subjects presented a 2.6‐fold and an 11‐fold increase of the levels of TFAM protein and TFAM mRNA, respectively. The increased expression of TFAM was associated to the doubling of NRF‐1 DNA‐binding affinity and to a 6‐fold increase of NRF‐1 mRNA level. The upregulation of TFAM and NRF‐1, in aged skeletal muscle, appears involved in the pathway leading to the age‐related increase of mitochondrial DNA content.