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Expression of membrane C1q in human monocyte‐derived macrophages is developmentally regulated and enhanced by interferon‐γ
Author(s) -
Kaul Marcus,
Loos Michael
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02592-3
Subject(s) - cd14 , phagocytosis , flow cytometry , microbiology and biotechnology , monocyte , interferon , in vitro , cell , biology , interferon gamma , reverse transcriptase , macrophage , chemistry , polymerase chain reaction , gene , immunology , biochemistry
The present study investigated when during ‘in vitro’ maturation macrophages (MΦ) express membrane C1q (mC1q), and whether cell activation affects expression and function of mC1q. Although C1q mRNA was repeatedly detected in freshly isolated monocytes using reverse transcriptase‐polymerase chain reaction, C1q protein was observed only in developing MΦ from day 1 to 4 on using immunodetection and flow cytometry. However, the quantity of mC1q and other MΦ membrane proteins differed strikingly in cells from different donors. We report here for the first time that CD14 + and CD14 − mC1q‐bearing MΦ can develop, and that interferon‐γ increases mC1q display at the cell surface, and mC1q‐mediated phagocytosis.