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An NMR‐based metabonomic approach to the investigation of coelomic fluid biochemistry in earthworms under toxic stress
Author(s) -
Bundy J.G,
Osborn D,
Weeks J.M,
Lindon J.C,
Nicholson J.K
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02582-0
Subject(s) - malonate , chemistry , earthworm , formate , coelom , biochemistry , chromatography , biology , anatomy , ecology , catalysis
The endogenous metabolites of the coelomic fluid of the earthworm Eisenia veneta were characterised using high‐resolution one‐dimensional and two‐dimensional 1 H nuclear magnetic resonance spectroscopy. Signals from common organic acids, such as acetate, fumarate, malonate, malate, formate, and succinate, were identified together with adenosine and nicotinamide mononucleotide. The potential use of this information as a baseline dataset for future toxicological or physiological studies was demonstrated by a metabonomic analysis: a series of earthworms were dosed with the model compound 3‐fluoro‐4‐nitrophenol, and toxic effects followed by multivariate analysis of the spectral data of the coelomic fluid. Relative concentrations of acetate and malonate were decreased in the dosed worms compared to the controls.