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Non‐invasive visualization of sperm mitochondria behavior in transgenic mice with introduced green fluorescent protein (GFP)
Author(s) -
Shitara Hiroshi,
Kaneda Hideki,
Sato Akitsugu,
Iwasaki Kuniko,
Hayashi Jun-Ichi,
Taya Choji,
Yonekawa Hiromichi
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02574-1
Subject(s) - green fluorescent protein , mitochondrion , sperm , cytoplasm , transgene , biology , genetically modified mouse , microbiology and biotechnology , mitochondrial dna , fluorescence microscope , confocal microscopy , fluorescence , biochemistry , gene , genetics , physics , quantum mechanics
Using high sensitive polymerase chain reaction (PCR), we previously demonstrated that selective elimination of sperm mitochondrial DNA occurred during early embryogenesis in mouse. To analyze the process morphologically in more detail, a non‐invasive, real‐time observation of sperm mitochondria was used. Transgenic mice that express green fluorescent protein (GFP) exclusively in mitochondria (mtGFP‐tg mice) were generated. The fluorescence in mtGFP‐tg mice was strong and stable enough to carry out repeated observations under confocal laser scanning microscopy. In these mtGFP‐tg mice it was revealed that the sperm mitochondria were selectively eliminated from egg cytoplasm during the two‐cell stage of early embryogenesis. Therefore, mtGFP‐tg mice should contribute to studies on sequential or repeated analysis of mitochondria.