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The fission yeast meiotic regulator Mei2p undergoes nucleocytoplasmic shuttling
Author(s) -
Sato Masamitsu,
Shinozaki-Yabana Satoko,
Yamashita Akira,
Watanabe Yoshinori,
Yamamoto Masayuki
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02567-4
Subject(s) - schizosaccharomyces pombe , meiosis , microbiology and biotechnology , nucleus , schizosaccharomyces , rna , mitosis , cell nucleus , chemistry , nuclear transport , biology , yeast , biophysics , genetics , saccharomyces cerevisiae , gene
Schizosaccharomyces pombe Mei2p is an RNA‐binding protein that switches the cell cycle from mitotic to meiotic. Mei2p forms a unique dot in the nucleus prior to meiosis I, aided by a non‐coding RNA molecule termed meiRNA. Here we show that Mei2p intrinsically undergoes nucleocytoplasmic shuttling. Artificial acceleration of nuclear migration of Mei2p advances nuclear dot formation, but meiRNA does not appear to promote the dot formation by modulating the migration rate of Mei2p into the nucleus. Rather, this RNA is likely to facilitate the assembly of Mei2p into a dot structure and trap the protein as such in the nucleus.