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Bidirectional activity and orientation‐dependent specificity of the rat aldolase C promoter in transgenic mice
Author(s) -
Phan Dinh Tuy Françoise,
Porteu Arlette,
Kahn Axel,
Skala Henriette
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02540-6
Subject(s) - chloramphenicol acetyltransferase , ap 1 transcription factor , transgene , microbiology and biotechnology , reporter gene , biology , transcription (linguistics) , aldolase a , acetyltransferase , gene , genetically modified mouse , promoter , transcription factor , gene expression , genetics , enzyme , biochemistry , linguistics , philosophy , acetylation
We previously reported that the rat aldolase C 115 bp promoter is sufficient to ensure the brain specific expression of the chloramphenicol acetyltransferase reporter gene in transgenic mice. We identify in a further reduced 84 bp promoter several putative binding sites for the transcriptional factors Sp1, USF, AP1, and AP2. Deletion or mutation of these partially overlapping binding sites results in inactivation of the cognate transgenes. Moreover, we show that the 115 bp sequence is able to direct bidirectional transcription in vivo but, surprisingly, transcriptional activity in the opposite direction is no more brain specific.