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Characterization of two genes, Sig1 and Sig2 , encoding distinct plastid σ factors 1 in the moss Physcomitrella patens : phylogenetic relationships to plastid σ factors in higher plants
Author(s) -
Hara Keishi,
Morita Masakatsu,
Takahashi Rieko,
Sugita Mamoru,
Kato Seiji,
Aoki Setsuyuki
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02530-3
Subject(s) - physcomitrella patens , plastid , biology , gene , complementary dna , genetics , phylogenetic tree , genome , transcription factor , chloroplast , mutant
We isolated the cDNA for a σ factor from the moss Physcomitrella patens , which possesses unusually large N‐terminal extension and the conserved subdomains 1.2–4.2. Phylogenetic analyses indicated that this novel σ factor and PpSIG1* 2 , a plastid σ factor previously identified from Physcomitrella , were classified into SigA and SigB groups, two major classes of higher plant plastid σ factors, respectively. According to the nomenclature recently proposed, we renamed PpSIG1* into PpSIG2, and named the novel σ factor PpSIG1. A transient expression assay using a green fluorescent protein showed that the N‐terminal region of PpSIG1 acts as a chloroplast‐targeting signal. Reverse transcription‐PCR experiments showed that light induces the expression of the Sig1 and Sig2 genes encoding PpSIG1 and PpSIG2, respectively. Thus, PpSIG1 and PpSIG2 are likely plastid σ factors regulating plastid gene expression in response to light signals.