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A deeper investigation on carbohydrate cycling in Sinorhizobium meliloti
Author(s) -
Gosselin Isabelle,
Wattraint Olivier,
Riboul David,
Barbotin Jean-Noël,
Portais Jean-Charles
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02518-2
Subject(s) - sinorhizobium meliloti , biochemistry , carbohydrate , periplasmic space , pentose phosphate pathway , assimilation (phonology) , cycling , metabolic pathway , bacteria , chemistry , fructose , polysaccharide , biology , glycolysis , metabolism , gene , mutant , escherichia coli , genetics , history , linguistics , philosophy , archaeology
Recycling of triose‐phosphate and pentose‐phosphate was previously reported on glucose in Sinorhizobium meliloti , a polysaccharide‐synthesizing bacterium, but the metabolic basis of such processes remained unclear. In this work, we have used 13 C‐labelling strategies to demonstrate that carbohydrate cycling in this organism is independent of the gluconate bypass, the alternative pathway for glucose assimilation involving its periplasmic oxidation into gluconate. Furthermore, carbohydrate cycling in S. meliloti is also observed on fructose, making the situation in this bacterium significantly different from that depicted for alginate‐synthesizing species.