Premium
Influence of oocyte nuclei on demethylation of donor genome in cloned bovine embryos
Author(s) -
Kang Yong-Kook,
Koo Deog-Bon,
Park Jung-Sun,
Choi Young-Hee,
Lee Kyung-Kwang,
Han Yong-Mahn
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02514-5
Subject(s) - biology , cpg site , dna demethylation , demethylation , dna methylation , methylation , genome , bisulfite , bisulfite sequencing , embryo , bovine genome , genetics , microbiology and biotechnology , dna sequencing , ploidy , dna , gene , gene expression
We recently demonstrated that satellite regions exhibit an aberrant DNA methylation in cloned bovine embryos. Here, we examined, using bisulfite‐sequencing technology, whether the inefficient demethylation of cloned donor genomes could be rescued by the presence of oocytic nuclei. Both Aci I digestion and sequencing analyses showed that satellite sequence was demethylated more efficiently in cloned tetraploid blastocysts than in diploid clones. When methyl‐CpG density (the number of methyl‐CpG sites per string) was scored, a significant decrease was observed in tetraploids ( P <0.001). These results suggest that unknown mechanisms provided by oocytic nuclei could assist the demethylation of satellite sequences in tetraploid clones.