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Identification of novel mast cell genes by serial analysis of gene expression in cord blood‐derived mast cells
Author(s) -
Kuramasu Atsuo,
Kubota Yasuhiko,
Matsumoto Kenji,
Nakajima Toshiharu,
Sun Xu-Ming,
Watanabe Takehiko,
Saito Hirohisa,
Ohtsu Hiroshi
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02477-2
Subject(s) - mast cell , gene , cord blood , mast (botany) , gene expression , biology , identification (biology) , microbiology and biotechnology , immunology , genetics , botany
The gene expression profile of human cord blood‐derived mast cells (MCs) was investigated using serial analysis of gene expression (SAGE). A total of 22 914 tags, representing 9181 unique transcripts, were sequenced. By selecting tags that were detected more frequently in MCs than in other tissues, genes characteristic of MCs were enriched. Reverse transcription‐PCR and the high‐density oligonucleotide array hybridization confirmed the validity of our SAGE result. About 70% of the selected genes were previously uncharacterized. Northern blot analysis showed the MC‐specific expression of selected genes. This inventory will be useful to identify novel genes with important functions in MCs.