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Phosphatidylserine delivery to endoplasmic reticulum‐derived vesicles of plant cells depends on two biosynthetic pathways
Author(s) -
Vincent Patrick,
Maneta-Peyret Lilly,
Cassagne Claude,
Moreau Patrick
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02475-9
Subject(s) - endoplasmic reticulum , phosphatidylserine , vesicle , biochemistry , enzyme , serine , microbiology and biotechnology , chemistry , biology , membrane , phospholipid
Vesicles formed from endoplasmic reticulum (ER) by a cell‐free system of leek cells ( Allium porrum ) are enriched in phosphatidylserine (PS), especially species containing very long chain fatty acids (VLCFA, at least 20 carbon atoms). In plant cells, PS is formed either by PS synthase or the serine exchange enzyme, although it is not known which pathway(s) contribute(s) to PS delivery in the ER‐derived vesicles (EV), nor to what extent this occurs. Taking advantage of a cell‐free system, we have shown that PS enrichment originates mainly from the serine exchange enzyme which is the only pathway that synthesizes the VLCFA‐PS species. On the other hand, both enzymes synthesize PS with long chain fatty acids (up to 18 carbon atoms), but these species are given to the EV by PS synthase.