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Overlapping CRE and E‐box promoter elements can independently regulate COX‐2 gene transcription in macrophages
Author(s) -
Mestre Juan R.,
Rivadeneira David E.,
Mackrell Peter J.,
Duff Michael,
Stapleton Philip P.,
Mack-Strong Vivian,
Maddali Sirish,
Smyth Gordon P.,
Tanabe Tadashi,
Daly John M.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02422-x
Subject(s) - transcription (linguistics) , response element , promoter , transcription factor , e box , microbiology and biotechnology , caat box , biology , gene , dna binding protein , gene expression , genetics , philosophy , linguistics
Macrophage cyclooxygenase‐2 (COX‐2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E‐box was investigated. Nuclear proteins bound both the CRE and E‐box, which synergized with other promoter elements to induce COX‐2 transcription. Endotoxin induced binding of nuclear proteins to the CRE and E‐box and each element independently induced higher COX‐2 transcription levels than the overlapping CRE/E‐box. Transcription factors associated with the CRE binding complex included c‐Jun and CRE binding protein and with the E‐box binding complex USF‐1; their overexpression significantly induced COX‐2 transcription. Therefore, both CRE and E‐box promoter elements regulate COX‐2 transcription in macrophages.