Premium
An integrated approach in the discovery and characterization of a novel nuclear protein over‐expressed in liver and pancreatic tumors
Author(s) -
Choong Meng Ling,
Tan Li Kiang,
Lo Siaw Ling,
Ren Ee-Chee,
Ou Keli,
Ong Shao-En,
Liang Rosa C.M.Y.,
Seow Teck Keong,
Chung Maxey C.M.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02409-7
Subject(s) - biology , complementary dna , microbiology and biotechnology , hepatocellular carcinoma , gene , proteome , peptide sequence , open reading frame , computational biology , cancer research , genetics
An integrated approach in protein discovery through the use of multidisciplinary tools was reported. A novel protein, Hcc‐1, was identified by analysis of the hepatocellular carcinoma (HCC)‐M cell proteome. The assembled EST sequence of the 210 amino acid novel protein was subsequently confirmed by rapid amplification of cDNA ends (RACE). A total of 687 bp at the 5′ untranslated region of Hcc‐1 was identified. Promoter activity and several upstream open reading frames (uORFs) were demonstrated at this region. Bioinformatics prediction showed that the first 42 amino acids of the protein is a SAP domain with sequence matches to hnRNP from various vertebrate species. The Hcc‐1 protein was localized to the cell nucleus while the gene was localized to chromosome 7q22.1. Hcc‐1 cDNA level was increased in pancreatic adenocarcinoma. The level was also increased in well‐differentiated hepatocellular carcinoma but decreases as the carcinoma progressed to a poorly differentiated stage.