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Two KaiA‐binding domains of cyanobacterial circadian clock protein KaiC
Author(s) -
Taniguchi Yasuhito,
Yamaguchi Akihiro,
Hijikata Atsushi,
Iwasaki Hideo,
Kamagata Kyoko,
Ishiura Masahiro,
Go Mitiko,
Kondo Takao
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02408-5
Subject(s) - biology , circadian rhythm , circadian clock , yeast , genetics , microbiology and biotechnology , gene , neuroscience
kaiABC , a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA‐binding domains (C KABD1 and C KABD2 ) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of C KABD –KaiA interactions assayed by the yeast two‐hybrid system. Thus, interaction between KaiA and KaiC through C KABD1 and C KABD2 is likely important for circadian timing in the cyanobacterium.