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Conventional protein kinase C isoforms and cross‐activation of protein kinase A regulate cardiac Na + current
Author(s) -
Shin Hyeon-Gyu,
Murray Katherine T
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02380-8
Subject(s) - protein kinase c , gene isoform , internalization , protein kinase a , chemistry , kinase , microbiology and biotechnology , biochemistry , biology , cell , gene
We tested the hypothesis that specific isoforms of protein kinase C (PKC) are responsible for modulation of Na + current ( I Na ) derived from the human cardiac Na + channel using activators and inhibitors selective for specific PKCs. Experimental results demonstrated that I Na suppression was mediated by activation of conventional PKCs (cPKCs) and possibly resulted from channel internalization. In the presence of cPKC inhibition, phorbol ester application unexpectedly increased Na + current, an effect eliminated by inhibition of protein kinase A. These findings demonstrate complex modulation of cardiac I Na by protein kinases and provide further evidence that PKC isoforms have distinct protein targets.