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Molecular characterization of human UDP‐glucuronic acid/UDP‐ N ‐acetylgalactosamine transporter, a novel nucleotide sugar transporter with dual substrate specificity
Author(s) -
Muraoka Masatoshi,
Kawakita Masao,
Ishida Nobuhiro
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02358-4
Subject(s) - nucleotide sugar , biochemistry , glucuronic acid , transporter , chemistry , glycosyltransferase , heterologous expression , yeast , nucleotide , gene , microbiology and biotechnology , biology , recombinant dna , polysaccharide
A novel human nucleotide sugar transporter (NST) which transports both UDP‐glucuronic acid (UDP‐GlcA) and UDP‐ N ‐acetylgalactosamine (UDP‐GalNAc) has been identified, cloned and characterized. The strategy for the identification of the novel NST involved a search of the expressed sequence tags database for genes related to the human UDP‐galactose transporter‐related isozyme 1, followed by heterologous expression of a candidate gene (hUGTrel7) in Saccharomyces cerevisiae and biochemical analyses. Significantly more UDP‐GlcA and UDP‐GalNAc were translocated from the reaction medium into the lumen of microsomes prepared from the hUGTrel7‐expressing yeast cells than into the control microsomes from cells not expressing hUGTrel7. The possibility that this transporter participates in glucuronidation and/or chondroitin sulfate biosynthesis is discussed.