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Diacylglycerol effects on phosphatidylinositol‐specific phospholipase C activity and vesicle fusion
Author(s) -
Villar Ana V,
Goñi Félix M,
Alonso Alicia
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02333-x
Subject(s) - diacylglycerol kinase , phosphatidylinositol , vesicle , phospholipase c , lipid bilayer fusion , chemistry , fusion , diglyceride , phosphoinositide phospholipase c , phospholipase , vesicle fusion , biochemistry , phosphatidylinositol 4,5 bisphosphate , liposome , enzyme , protein kinase c , membrane , synaptic vesicle , signal transduction , linguistics , philosophy
Diacylglycerol increased the hydrolytic activity of phosphatidylinositol‐specific phospholipase C on large unilamellar vesicles containing 5–40% phosphatidylinositol. Moreover, diacylglycerol increased the rate and extent of vesicle fusion (contents mixing) induced by the enzyme. Kinetic studies of intervesicular lipid mixing revealed that fusion was limited by the frequency of contacts involving two diacylglycerol‐rich domains.