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Characterization of a novel human putative mitochondrial transporter homologous to the yeast mitochondrial RNA splicing proteins 3 and 4
Author(s) -
Li Fang-Yuan,
Nikali Kaisu,
Gregan Juraj,
Leibiger Ingo,
Leibiger Barbara,
Schweyen Rudolf,
Larsson Catharina,
Suomalainen Anu
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02319-5
Subject(s) - biology , rna splicing , hspa9 , alternative splicing , genetics , gene , mitochondrial carrier , transmembrane protein , mitochondrion , mitochondrial dna , exon , rna , peptide sequence , receptor , escherichia coli , bacterial outer membrane
We report here a novel human gene, hMRS3 / 4 , encoding a putative mitochondrial transporter structurally and functionally homologous to the yeast mitochondrial RNA splicing proteins 3 and 4. These proteins belong to the family of mitochondrial carrier proteins (MCF) and are likely to function as solute carriers. hMRS3 / 4 spans ∼10 kb of genomic DNA on chromosome 10q24 and consists of four exons that encode a 364‐aa protein with six transmembrane domains. A putative splice variant, encoding a 177‐aa protein with three transmembrane domains, was also identified. hMRS3/4 has a well‐conserved signature sequence of MCF and is targeted into the mitochondria. When expressed in yeast, hMRS3 / 4 efficiently restores the mitochondrial functions in mrs3 o mrs4 o knock‐out mutants. Ubiquitous expression in human tissues and a well‐conserved structure and function suggest an important role for hMRS3/4 in human cells.