Premium
Immunocytological localization of two plant fatty acid desaturases in the endoplasmic reticulum
Author(s) -
Dyer John M.,
Mullen Robert T.
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02315-8
Subject(s) - endoplasmic reticulum , calreticulin , subcellular localization , cytosol , membrane , biochemistry , epitope , chemistry , cytoplasm , microbiology and biotechnology , microsome , biology , enzyme , antibody , genetics
The subcellular location of two integral membrane‐bound fatty acid desaturases (Fads), Fad2 and Fad3, was elucidated by immunofluorescence microscopic analyses of tobacco suspension cells transiently transformed with different epitope‐tagged versions of the enzymes. Both myc‐ or hemagglutinin‐tagged Fad2 and Fad3 localized to the same region of the endoplasmic reticulum (ER), as evidenced by their co‐localization with the ER lumenal protein calreticulin. Results from differential permeabilization experiments revealed that the N‐termini of both epitope‐tagged Fad2 and Fad3 were exposed on the cytosolic side of ER membranes. These data define the subcellular location and topological orientation of plant desaturases in ER membranes.