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A sequence motif responsible for ER export and surface expression of Kir2.0 inward rectifier K + channels
Author(s) -
Stockklausner C,
Ludwig J,
Ruppersberg J.P,
Klöcker N
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02286-4
Subject(s) - endoplasmic reticulum , golgi apparatus , er retention , cytoplasm , inward rectifier potassium ion channel , microbiology and biotechnology , potassium channel , secretory pathway , chemistry , biology , biophysics , biochemistry , ion channel , gene , receptor , mutant
Integral membrane proteins are sorted via the secretory pathway. It was proposed that this pathway is non‐selective provided that the cargo protein is properly assembled and lacks an endoplasmic reticulum (ER) retention signal. However, recent experimental evidence suggests that efficient export of proteins from the ER to the Golgi complex is not simply a default pathway. Here we demonstrate a novel sequence motif (FxYENEV) in the cytoplasmic C‐terminus of mammalian inward rectifier potassium (Kir) channels which determines ER export. This motif is found to be both necessary and sufficient for efficient export from the ER that eventually leads to efficient surface expression of Kir2.1 channels.