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Down‐regulation of peroxisome proliferator‐activated receptor‐γ gene expression by sphingomyelins
Author(s) -
Al-Makdissy Nehmann,
Bianchi Arnaud,
Younsi Mohamed,
Picard Evelyne,
Valet Philippe,
Martinet Nadine,
Dauça Michel,
Donner Mireille
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02278-5
Subject(s) - peroxisome , adipocyte , peroxisome proliferator activated receptor , adipose tissue , medicine , receptor , endocrinology , fatty acid , chemistry , sphingomyelin , peroxisome proliferator activated receptor gamma , adipocyte protein 2 , microbiology and biotechnology , membrane , biology , biochemistry
We recently demonstrated that the sphingomyelin (SM) content of adipocyte membranes was negatively correlated with the expression of peroxisome proliferator‐activated receptor‐γ (PPARγ) in the subcutaneous adipose tissue of obese women with variable degrees of insulin resistance. We have now investigated whether SM really does have an impact on the expression of PPARγ in 3T3‐F442A adipocytes. Adding SM to the culture medium for 24 h caused a significant increase in SM content of adipocyte membranes and an acyl chain length‐dependent decrease in the levels of PPARγ mRNA and protein. The longer the acyl chain of the fatty acid of SM, the greater was the decrease in PPARγ. These data suggest that the nature of the fatty acid is important in the regulation of PPARγ by the SM pathway.