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A cis ‐dominant cyclic nucleotide‐dependent regulatory domain in the 3′‐untranslated region of Na + /glucose cotransporter (SGLT1) mRNA
Author(s) -
Loflin Paul,
Lever Julia E
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02260-8
Subject(s) - cotransporter , untranslated region , messenger rna , nucleotide , chemistry , function (biology) , microbiology and biotechnology , biology , gene , biochemistry , organic chemistry , sodium
A 122 nt uridine‐rich sequence (URE) in the Na + /glucose cotransporter (SGLT1) mRNA 3′‐untranslated region is critical for cAMP‐dependent message stabilization. Its function was investigated in LLC‐PK 1 cells stably expressing β‐globin reporter transcripts. Insertion of the SGLT1 URE downstream from an unrelated destabilizing sequence, the c‐ fos ARE, evoked cAMP‐dependent message stabilization. Stabilization was blocked by a substitution mutation within the SLGT1 URE. These observations indicate that the SGLT1 URE is sufficient to transmit cAMP‐dependent, cis ‐dominant mRNA stabilization in the presence of appropriate trans ‐acting factors and appears to function independently of the nature of the destabilizing domain.