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Decreased antioxidant defense during replicative aging of the yeast Saccharomyces cerevisiae studied using the ‘baby machine’ method
Author(s) -
Grzelak Agnieszka,
Skierski Janusz,
Bartosz Grzegorz
Publication year - 2001
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(01)02244-x
Subject(s) - saccharomyces cerevisiae , percoll , yeast , superoxide dismutase , glutathione , antioxidant , differential centrifugation , centrifugation , biochemistry , chemistry , oxidative stress , biology , enzyme
Replicatively senescent cells of Saccharomyces cerevisiae were obtained using the ‘baby machine’ method by immobilizing cells on CovaLink™ NH 2 plates and allowing them to divide while exchanging medium and removing daughter cells. Centrifugation in a Percoll density gradient was employed for further purification of replicatively old yeast cells. Comparison of senescent cells showing more than 20 bud scars with cells from early stationary culture demonstrated a significant reduction of total and reduced glutathione and decrease of superoxide dismutase activity during replicative aging of yeast cells.